Citômetro de fluxo CytoFLEX

O citómetro de fluxo CytoFLEX, a primeira introdução à plataforma CytoFLEX, fornece o desempenho necessário num sistema fácil de usar, permitindo que o utilizador se centre na ciência e não se preocupe com a instrumentação. A sua sensibilidade e resolução superiores em todas as configurações dão-lhe vantagem sobre outros sistemas de citometria com quatro vezes mais o seu tamanho.•

 

Características

Excelente sensibilidade

Versatilidade da plataforma

Manutenção reduzida

Automação ativada

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Advancements in MESF Beads for Enhanced Fluorescence Quantification in Flow Cytometry The document discusses advancements in the development of MESF (Molecules of Equivalent Soluble Fluorochrome) beads for improved fluorescence quantification in flow cytometry. It highlights the importance of MESF beads in standardizing fluorescence measurements across instruments and experiments. The research introduces new nano-sized MESF beads incorporating various fluorescent dyes such as Pacific Blue®, SuperNova, FITC, PE, and APC. These beads are designed to have low background fluorescence and known dye content, making them suitable for small particle flow cytometry. Preliminary testing using the CytoFLEX nano Flow Cytometer demonstrated their potential for accurate quantification of dye molecules per bead. The study establishes a reliable methodology for MESF value calculation and shows how these beads can be used to determine fluorescence detection limits in biological samples. Although not yet commercially available, the new MESF beads are expected to become essential tools for instrument calibration and assay standardization in advanced flow cytometry applications.

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